EXPLORING FLUORESCENCE MICROSCOPY AND SPECTROSCOPY
Fluorescence microscopy and spectroscopy are pivotal techniques in modern scientific research, offering unparalleled insights into the molecular and cellular world. By exploiting the natural fluorescence of materials or using fluorescent markers, these methods allow researchers to visualize and analyze the intricate details of biological samples, chemical compounds, and materials science.
Precision and Performance
Optical filters are at the heart of fluorescence microscopy and spectroscopy, crucial for guiding light from source to sensor with precision. At Omega Optical, we specialize in designing filters with extremely steep edges for cut-on and cut-off transitions, ensuring precise wavelength selection. Our filters provide deep blocking in the ultraviolet, visible, and infrared regions, enhancing signal clarity and reducing background noise. This high level of performance is essential for capturing detailed and accurate spectral data.
At Omega Optical, we are dedicated to advancing fluorescence microscopy and spectroscopy through our innovative optical solutions. Our expertise in optical filters, dichroic mirrors, diffraction gratings, and specialty mirror coatings ensures that your research is supported by the highest quality components, enabling you to guide light from source to sensor with unmatched precision.
Discover how Omega Optical can enhance your fluorescence research. Contact us today to learn more about our products and services.
Splitting and Combining Light with Accuracy
Dichroic mirrors play a key role in fluorescence applications by selectively reflecting and transmitting specific wavelengths. These mirrors are designed to split light efficiently, directing the desired wavelengths to the detector while reflecting others away. Our dichroic mirrors are engineered to achieve high performance across a wide spectral range, ensuring optimal separation and combination of light for fluorescence imaging and analysis.
At Omega Optical, we are dedicated to advancing fluorescence microscopy and spectroscopy through our innovative optical solutions. Our expertise in optical filters, dichroic mirrors, diffraction gratings, and specialty mirror coatings ensures that your research is supported by the highest quality components, enabling you to guide light from source to sensor with unmatched precision.
Discover how Omega Optical can enhance your fluorescence research. Contact us today to learn more about our products and services.
Unraveling Complex Spectra
Diffraction gratings are essential for dispersing light into its constituent wavelengths in fluorescence spectroscopy. Our diffraction gratings are designed to provide high resolution and efficiency, allowing for the detailed analysis of complex spectral data. These gratings are integral in applications where precise wavelength discrimination is necessary, such as in genomic sequencing and advanced fluorescence techniques.
At Omega Optical, we are dedicated to advancing fluorescence microscopy and spectroscopy through our innovative optical solutions. Our expertise in optical filters, dichroic mirrors, diffraction gratings, and specialty mirror coatings ensures that your research is supported by the highest quality components, enabling you to guide light from source to sensor with unmatched precision.
Discover how Omega Optical can enhance your fluorescence research. Contact us today to learn more about our products and services.
Enhancing Reflectivity and Durability
Specialty mirror coatings are critical for maximizing the reflectivity and durability of optical components used in fluorescence microscopy and spectroscopy. Our proprietary coatings ensure that mirrors maintain high reflectivity over a broad spectral range, from ultraviolet to infrared. These coatings also enhance the longevity and reliability of the mirrors, making them suitable for demanding research environments.
At Omega Optical, we are dedicated to advancing fluorescence microscopy and spectroscopy through our innovative optical solutions. Our expertise in optical filters, dichroic mirrors, diffraction gratings, and specialty mirror coatings ensures that your research is supported by the highest quality components, enabling you to guide light from source to sensor with unmatched precision.
Discover how Omega Optical can enhance your fluorescence research. Contact us today to learn more about our products and services.
Fluorescence microscopy and spectroscopy are utilized in a wide range of applications, each benefiting from the precision and reliability of our optical components:
- Flow Cytometry: Enables the analysis of physical and chemical characteristics of particles in a fluid as they pass through at least one laser.
- Genomic Sequencing: Utilizes fluorescent markers to read and analyze genetic information with high accuracy.
- Forster Resonance Energy Transfer (FRET): Studies molecular interactions by measuring energy transfer between fluorophores.
- Live Cell Imaging: Observes cellular processes in real-time, providing insights into dynamic biological systems.
- Drug Discovery: Screens and identifies potential drug candidates through high-throughput fluorescence assays.
- Environmental Monitoring: Detects and quantifies pollutants using fluorescence-based sensors.
At Omega Optical, we are dedicated to advancing fluorescence microscopy and spectroscopy through our innovative optical solutions. Our expertise in optical filters, dichroic mirrors, diffraction gratings, and specialty mirror coatings ensures that your research is supported by the highest quality components, enabling you to guide light from source to sensor with unmatched precision.
Discover how Omega Optical can enhance your fluorescence research. Contact us today to learn more about our products and services.
Explore the Features
full optical capabilities
Learn more about the full suite of capabilities which are trusted by leading Global OEM’s and their Start-up challengers to deliver the right photons, to the right place, at the right time.
Specification considerations
Key Parameters
Peak Transmission is the maximum transmission of the filter (%).
Center Wavelength (CWL) is the midpoint of the transmitting region (nm).
Full-width half-max (FWHM) describes the width of the transmitting region at half of the maximum transmission value (nm). Because of the physics of thin-film interference filters, FWHM generally increases with wavelength. It can be calculated using a percentage of the center wavelength.
Tolerancing
Cut-on and Cut-off wavelengths can be used instead of FWHM and CWL to designate the edges of the filter's transmitting region (nm).
Pass band ripple describes the flatness of the transmitting region (%). Some narrow bandpass filters and older designs have a peaked pass band shape instead of a flat top. This depends on filter design. Typically flat-top designs require more layers and greater cost.
Blocking
Blocking specifications determine the wavelength range (nm) and blocking (OD) of unwanted light. Typically the wavelength range is dictated by the response curve of your detector. However, extended blocking is not required for some applications (for example laser-cleanup or Raman filters) where unwanted signals are only present in a limited wavelength range. Optical density (OD) is a log scale relating the transmitted to incident light.
OD=2-log(%T)
Edge Refinement
Edge Steepness describes the transition from blocking to transmission in more detail (nm).
Key Supporting Capabilities
Solution
Design & Engineering
Our commitment to finding the right solutions for difficult problems means that we are capable of doing what other engineers cannot. Some say we bend physics and solve the unsolvable. Can we? Maybe. What we know we can do is this: We ask questions and we dig, because sometimes the solutions people believe they need only scratch the surface.
Solution
Optical Coatings
Omega coating heritage dates back to 1936, and spans a wide range of technologies: Sunglasses to space-borne sun-visors; halogen light bulbs to COVID-fighting UV-C lamps, supermarket scanners to modern printers, astronomical mirrors to prestigious art installations to entertainment optics: if an application requires a precision or decorative coatings we surely have a proven solution.
Solution
Grating Manufacturing
We have the diffractive optics trifecta of multiple Ruling Engines, Holographic Mastering, and Lithographic exposure to create diffractive elements. Our in-house design and manufacturing processes provide standard or custom gratings for a range of applications, in both Original and Replicated formats.
Eyebrow
Specialty Patterning & Cutting
We produce more than just circles and rectangular shaped components. Challenge us to produce a complex shape or pattern demanded by your application.
